PF20 gene product contains WD repeats and localizes to the intermicrotubule bridges in Chlamydomonas flagella.
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چکیده
منابع مشابه
PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella
Several studies have indicated that the central pair of microtubules and their associated structures play a significant role in regulating flagellar motility. To begin a molecular analysis of these components we have generated central apparatus-defective mutants in Chlamydomonas reinhardtii using insertional mutagenesis. One paralyzed mutant recovered in our screen, D2, is an allele of a previo...
متن کاملCDKL5 regulates flagellar length and localizes to the base of the flagella in Chlamydomonas
The length of Chlamydomonas flagella is tightly regulated. Mutations in four genes-LF1, LF2, LF3, and LF4-cause cells to assemble flagella up to three times wild-type length. LF2 and LF4 encode protein kinases. Here we describe a new gene, LF5, in which null mutations cause cells to assemble flagella of excess length. The LF5 gene encodes a protein kinase very similar in sequence to the protein...
متن کاملIntermicrotubule Bridges in Mitotic Spindle Apparatus
Fine interconnections or "cross-bridges" have been observed between cytoplasmic microtubules in many different organisms (1, 3, 7, 9, 10, 12, 13) . Recently, similar bridges have been noted between the microtubules of the spindle apparatus in dividing plant and animal cells (4, 6, 7, 11, 14) . Increasing attention is being directed toward intertubule bridges, since they may play an important ro...
متن کاملChlamydomonas Flagella
Methods were developed for the isolation of Chlamydomonas flagella and for their fractionation into membrane, mastigoneme, "matrix," and axoneme components. Each component was studied by electron microscopy and acrylamide gel electrophoresis. Purified membranes retained their tripartite ultrastructure and were shown to contain one high molecular weight protein band on electrophoresis in sodium ...
متن کاملHaploinsufficiency for the murine orthologue of Chlamydomonas PF20 disrupts spermatogenesis.
PF20 was first identified in Chlamydomonas rheinhardtii as an essential component of the axoneme central apparatus. We discovered that the mouse Pf20 gene encodes two major transcripts (2.5 and 1.4 kb), which are expressed in different patterns during spermatogenesis, yielding proteins of 71 and 35 kDa, respectively. Both proteins contain contiguous WD repeats in their C termini. The meioticall...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1997
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.8.3.455